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The Zebrafish: Genetics, Genomics, and Informatics by H. William Detrich, III, Monte Westerfield, and Leonard I.

24 February 2017 adminCell Biology

By H. William Detrich, III, Monte Westerfield, and Leonard I. Zon (Eds.)

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Additional resources for The Zebrafish: Genetics, Genomics, and Informatics

Example text

Only mutation m1 is followed in subsequent generations for simplicity. Two F1 fish are intercrossed and an F2 family raised. Half the individuals of the F2 family are heterozygous for m1. F2 fish are intercrossed to make an F3-extended family (see text), composed of equal numbers of F3 fish from each of the F2 intercrosses of one family. One quarter of the F2 intercrosses are between m1 heterozygotes (boxed with hatched lines), producing m1 homozygotes (boxed with solid lines) in 25% of their F3 progeny.

Stemple, D. L. (2004). TILLING—a high-throughput harvest for functional genomics. Nat. Rev. Genet. 5, 145–150. Stuart, G. , McMurray, J. , and Westerfield, M. (1988). Replication, integration and stable germline transmission of foreign sequences injected into early zebrafish embryos. Development 103, 403–412. , and Hopkins, N. (2001). vhnf1, the MODY5 and familial GCKD-associated gene, regulates regional specification of the zebrafish gut, pronephros, and hindbrain. Genes Dev. 15, 3217–3229. Trofatter, J.

B. (2004). A highthroughput method for indentifying ENU-induced point mutations in zebrafish. Methods Cell Biol. 77, 91–112. , Schier, A. , Neuhauss, S. C. , Stemple, D. , Stainier, D. Y. , and Boggs, C. (1996). A genetic screen for mutations aVecting embryogenesis in zebrafish. Development 123, 37–46. FlyBase Consortium(2002). The FlyBase database of the Drosophila genome projects and community literature. Nucleic Acids Res. 30, 106–108. Foster, J. , Dominguez-Steglich, M. , Weller, P. , Young, I.

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