By Alan Smith
Describing the medical and advertisement functions of microbial recombinant DNA expertise, this notable, single-source reference bargains cutting-edge studies of gene expression within the most crucial sessions of recombinant microorganisms-providing various examples of the expression of homologous genes or heterologous gene items. provides a special selection of security and regulatory issues from all over the world and addresses particular measures to be taken for large-scale business operations!
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Extra resources for Gene Expression in Recombinant Microorganisms
Sample text
Brownlie L, Stephenson JR, Cole JA. Effect of growth rateon plasmid maintenance by Escherichia coli HBlOl(pAT153). J Gen Microbiol 1990;136: 2471-2480. 7. Khosravi M, Webster DA, Stark BC. Presence of the bacterial hemoglobin gene improves a-amylase production of a recombinant Escherichia colistrain. Plasmid 1990; 24:190-194. 8. Edlin G, Tait RC, RodrigeuzRL. A bacteriophage X cohesive ends(cos)DNA fragment enhanm the fitness of plasmidcontaining bacteria growing in energylimited chemostats. Bio/Technology 1984; 2:251-254.
MRNA sequences that have intramolecular structures involving the SD or the initiation codon should be avoided. 2. A long (five to eight nucleotides) SD is favorable for translation. 3. The spacing between the SD and the initiation codon is important but depends on the length and sequence of the SD. As a result of the precise distance between the 3' end of the 16s rRNA and the binding sitefor the initiator tRNA inthe ribosome, longer spacing is more tolerable than shorter spacing. 4. AUG is most favorable as an initiation codon.
VII. Construction ofplasmid pBR327par, a completely sequenced, stable derivariveof pBR327 containing thepar locus of pSC101. Gene 1984; 28~119-122. 18. Tomizawa J-I, Itoh T. The importance of RNA secondary structure inColEl primer formation. Cell 1982; 31575-583. 19. Davison J. Mechanism of control of DNA replication and incompatibility in ColEl-type plasmids: A review. Gene 1984; 28:l-15. U). Tomizawa J-I. Control of ColEl plasmid replication:The process of binding of RNA I to the primer transcript.