By Kenworthy, Anne
Current themes in Membranes is certain towards scientists and researchers in biochemistry and molecular and mobile biology, offering the required membrane examine to aid them in studying the present kingdom of a selected box and in studying the place that box is heading. This quantity deals an up to the moment presentation of present wisdom within the box of Lipid Domains.
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Example text
Although the concentration used for GPMV isolation is w50-fold less than is used for fixation, significant cross-linking is still likely, and its effects on the organization of the membrane are unclear. In addition, there are more specific effects of the isolation chemicals that clearly have direct effects on proteins and membrane domains. , 2010). Additionally, formaldehyde and DTT appear to cooperatively cross-link membrane proteins to the amine-containing glycerophospholipids phosphatidylethanolamine (PE), resulting in extraction of proteins from the ordered domains (Levental, Grzybek, & Simons, 2011).
Tamm, L. K. (2001). Reconstituted syntaxin1A/SNAP25 interacts with negatively charged lipids as measured by lateral diffusion in planar supported bilayers. Biophysical Journal, 81(1), 266e275. , Cafiso, D. , & Tamm, L. K. (2011). Partitioning of synaptotagmin I C2 domains between liquid-ordered and liquid-disordered inner leaflet lipid phases. Biochemistry, 50(13), 2478e2485. , & Tamm, L. K. (2008). Coupling of cholesterol-rich lipid phases in asymmetric bilayers. Biochemistry, 47(7), 2190e2198.
Nature Chemical Biology, in press. CHAPTER TWO Giant Plasma Membrane Vesicles: Models for Understanding Membrane Organization Kandice R. edu Contents 1. Introduction 2. 6 Caveats of membrane model systems 3. 3 Critical fluctuations in GPMVs 4. 4 Alternative PM model systems 5. 5 Other uses of GPMVs 6. 009 25 © 2015 Elsevier Inc. All rights reserved. 26 27 28 28 30 31 31 33 34 34 35 36 37 37 39 39 40 40 41 44 45 j 26 Kandice R. Levental and Ilya Levental Abstract The organization of eukaryotic membranes into functional domains continues to fascinate and puzzle cell biologists and biophysicists.