By Dagmar Klostermeier, Christian Hammann
Whereas structure-function relationships of proteins were studied for a very long time, structural reviews of RNA face extra demanding situations. however, with the continual discovery of novel RNA molecules with key mobile services and of novel pathways and interplay networks, the necessity for structural details of RNA continues to be expanding. This quantity presents an advent into concepts to evaluate constitution and folding of RNA. each one bankruptcy explains the theoretical history of 1 procedure, and illustrates probabilities and obstacles in chosen program examples.
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Additional info for RNA Structure and Folding: Biophysical Techniques and Prediction Methods
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6). 45) Experiments at multiple temperatures allow for the direct distinction of a temperature-independent ΔCp (linear relationship between ΔH and T) or temperaturedependent ΔCp (nonlinear dependence of ΔH on T) and thus yield reliable values for ΔCp. 2 Differential scanning calorimetry In DSC, the two-chamber principle of the ITC is also employed; however, the sample cell containing the RNA (in the presence or absence of ligands) and the reference cell are closed (Fig. 4B). Practically, a DSC instrument measures the difference in power that is required to maintain the temperature constant between reference and sample cell.