By Mien-Chie Hung, Leaf Huang, Ernst Wagner
Gene move inside of people has been a drawback till approximately 10 years in the past. at the moment, it used to be came upon that viral vectors have been powerful vendors of "healthy genes" into sufferers' cells. the matter, besides the fact that, was once that viral vectors proved unnecessarily destructive to people: topics skilled inflamatory job and adverse immunological responses to the genes. Viral vectors have been additionally not able to satisfy the wishes of the pharmaceutical neighborhood: they weren't reproducible in large-scale proportions in within your means ways.Thus, study used to be undertaken to discover a more secure solution to move genes to sufferers with out jeopardizing the protection of the sufferer. And so non-viral vectors have been chanced on. This quantity offers a number of the non-viral vectors presently below improvement. even though now not methodologically orientated, it is going to give you the worthwhile information in the back of the improvement of the vectors. this knowledge will end up valuable to either researchers and clinicians. Key positive factors* offers state-of-the artwork advancements of nonviral vectors as instruments for contemporary molecular drugs* Covers every kind of nonviral vectors, from molecular constitution to healing applicationProvides a complete overview of artificial vectors* comprises contributions from significant investigators and major specialists within the box
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Example text
In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment. Proc. Natl. Acad. Sci. USA 87, 9568 – 9572. , and Yoshikawa, K. (1995). Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double-stranded DNA chain. FEBS Lett. 361, 277–281. , Scherer, M. , Crystal, R. G. (1992). Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intracheal plasmid-mediated gene transfer. Nucleic Acid Res.
The following is a summary of these vectors and delivery methods, which are discussed in greater detail in various chapters of this book. 1. , 1995). , 1998). For systemic administration, however, the plasmid DNA needs to be protected from degradation by endonucleases during delivery from the site of administration to the site of gene expression. 2. Gene Gun Shooting DNA into cells can be carried out by using a physical method such as bioballistic bombardment or gene gun. The gene gun uses gold particles coated with DNA, which are transferred to a mylar carrier sheet.
A sustained, cytoplasmic transgene expression system delivered by cationic liposomes. Biochem. Biophys. Res. Commun. 200, 1201–1206. , and Curiel, D. (1993). Direct in vivo gene transfer to airway epithelium employing adenovirus-polylysine-DNA complexes. Human Gene Ther. 4, 17–24. , Guttman, S. , and Minsky, A. (1993). Mode of formation and structural features of DNA-cationic liposome complexes used for transfection. Biochemistry 32, 7143 –7151. Glorioso, J. , Goins, W. , Meaney, C. , Fink, D. , and DeLuca, N.