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Methods of Enzymology. Small GTPases and Their Regulators by Balch W.E., Channing J.D., Hall A.

24 February 2017 adminCell Biology

By Balch W.E., Channing J.D., Hall A.

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Kato, and Y. Takai, Z Biol. Chem. 268, 23959 (1993). a9y. Miura, A. Kikuchi, T. Musha, S. Kuroda, H. Yaku, T. Sasaki, and Y. Takai, Z Biol. Chem. 268, 510 (1993). z0 K. Kishi, T. Sasaki, S. Kuroda, T. Itoh, and Y. Takai, J. Cell Biol. 120, 1187 (1993). 21 K. Takaishi, A. Kikuchi, S. Kuroda, K. Kotani, T. Sasaki, and Y. Takai, Mol. Cell. Biol. 13, 72 (1993). 22 K. Takaishi, T. Sasaki, M. Kato, W. Yamochi, S. Kuroda, T. Nakamura, M. Takeichi, and Y. Takai, Oncogene 9, 273 (1993). 23T. Nishiyama, T.

H. Pillinger, R. Staud, C. Volker, M. G. Rosenfeld, G. Weissmann, and J. B. Stock, Science 259, 977 (1993). 10M. H. Pillinger, C. Volker, J. B. Stock, G. Weissmann, and M. R. Philips, Z Biol. Chem. 269, 1486 (1994). al A. Boyum, Scand. J. Clin. Lab. Invest. 21 (Suppl 97), 77 (1968). [7] C A R B O X Y L M E T H Y L T R A N S F E R A S E IN N E U T R O P H I L S 51 conical tubes and the cells are pelleted (800 rpm × 10 min). 6 M NaC1 to restore isotonicity. Isolated neutrophils are washed, resuspended, and counted in cell buffer.

Biol. Chem. 266, 12639 (1991). 25p. Adamson, C. J. Marshall,A. Hall, and P. A. Tilbrook,J. BioL Chem. 267, 20033 (1992). 26y. Hori, A. Kikuchi,M. Isomura, M. Katayama,Y. Miura, H. Fujioka, K. Kaibuchi, and Y. Takai, Oncogene 6, 515 (1991). z7T. Mizuno,K. Kaibuchi,T. Yamamoto,M. Kawamura,T. Sakoda, H. Fujioka,Y. Matsuura, and Y. Takai, Proc. Natl. Acad. Sci. A. 88, 6442 (1991). 44 EXPRESSION AND PURIFICATION [6] is used as a substrate. 6% CHAPS. A plasmid for expression of GST-Rho GDI is constructed as follows.

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