By Joel D. Richter
mRNA Formation and serve as offers a compendium of thoughts geared solely towards the knowledge of RNA metabolism. it is going to be fairly beneficial simply because a couple of diverse organisms and platforms are hired. Key beneficial properties * Isolation and characterization of particular RNA binding proteins * RNA metabolism and linked regulatory proteins * RNA detection and localization * A genetic method of RNA functionality
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Extra info for mRNA Formation and Function
To remove the aggregates formed during dialysis, centrifuge the extract for 5 min at 13,000 rpm in a microcentrifuge at 4~ Distribute the supernatant in 20-/xl aliquots and freeze in liquid nitrogen. This is considered the depleted extract. Keep at -70~ The protein concentration of the depleted extracts usually varies between 70% and 80% that of the original extract. 3. Analysisof Splicing Factors 35 It is generally useful to elute the material bound to oligo(dT), both to use it as a source of partially purified U2AF and to regenerate and reutilize the column.
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