By Hartmann, Roland Karl
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Extra resources for Handbook of RNA biochemistry
Sample text
The same strategy may also be considered for the synthesis of large amounts of smaller RNAs. Chemical synthesis and purification of 10 mg of, for example, an RNA 15-mer by a commercial supplier can be quite expensive. In such a case, a cheaper alternative would be to transcribe the 15-mer sandwiched between two cis-cleaving ribozymes, resulting in posttranscriptional release of the 15-mer with uniform 5 and 3 ends. 4). If T7 RNAP is self-prepared according to the protocol described in this chapter, synthesis of 10 mg of a 15-mer will become quite affordable.
Sproat Chemconsilium GCV Jaarmarktstraat 48 2221 Heist-op-den-Berg Belgium Karolinska Institutet Department of Laboratory Medicine H¨alsov¨agen 7 SE 14157 Huddinge Sweden and List of Contributors Thomas Tuschl The Rockefeller University Howard Hughes Medical Institute Laboratory of RNA Molecular Biology 1230 York Avenue New York, NY 10065 USA Jernej Ule MRC Laboratory of Molecular Biology Department of Structural Studies Hills Road Cambridge CB2 0QH UK Henning Urlaub Max-Planck-Institute for Biophysical Chemistry Bioanalytical Mass Spectrometry Group Am Fassberg 11 37077 G¨ottingen Germany and C.
Pavlova Philipps-Universit¨at Marburg Faculty of Pharmacy Marbacher Weg 6 35037 Marburg Germany Dimitri Podkaminski University of W¨urzburg Institute for Molecular Infection Biology RNA Biology Group Josef-Schneider-Strasse 2 97080 W¨urzburg Germany Ying Poi Liu University of Amsterdam Laboratory of Experimental Virology Department of Medical Microbiology Center for Infection and Immunity Amsterdam (CINIMA) Academic Medical Center Meibergdreef 15, K3-113D 1105 AZ Amsterdam The Netherlands XLIX L List of Contributors Marco Preußner Justus-Liebig-Universit¨at Giessen Institut f¨ur Biochemie Heinrich-Buff-Ring 58 35392 Giessen Germany and Institute of MolecularBiology and Tumor Research Philipps-Universit¨at Marburg Emil-Mannkopff-Strasse 2 35032 Marburg Germany Werner G.