By Yu-Li Wang, D. Lansing Taylor and K.W. Jeon (Eds.)
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Extra resources for Fluorescence Microscopy of Living Cells in Culture Part A . Fluorescent Analogs, Labeling Cells, and Basic Microscopy
Sample text
The fluorescent bands are excised and the modified toxins eluted overnight with 20% acetic acid. On the basis of a molar extinction coefficient at 565 nm of 65,000 M-I cm-' of the TmRhd chromophore and the extinction coefficient of the toxin at 277 nm, the preparative method typically yields pure 3. FLUORESCENT ANALOGS OF TOXINS 39 1. PREPARATION OF THlOAMlDlNYLATED TOXIN cI)iHPcI-- + SCORPION TOXIN (SCTX) @;"fc"icHisH s c TX- 2-IYINOTHlOLANE sn 2. AFFINITY COLUMN PURIFICATION -D s- s-s-SCTX ti s-s-SCTX + R'SH + SH + SCTX SH t R ' S S R ' 3.
259,4812-4821. Kahn, C. , Baird, K. ,Jarrett, D. , andFlier, J. S. (1978). Proc. Acad. 75, 4209-4213. , andohnuma, H. (1976). Biochim. Biophys. Acfa351, 205-21 3. Keen, J. , Maxfield, F. , Hardegree, M. , and Habig, W. H. (1982). Proc. Acad. Sci. 79,2912-2916. 28 FREDERICK R. MAXFIELD Levitzki, A. (1985). In “Endocytosis” (I. Pastan and M. C. Willingham. ), pp. 45-68. Plenum, New York. Maxfield, F. R. (1985). In “Endocytosis” (I. Pastan and M. C. ), pp. 235 -257. Plenum, New York. Maxfield, F. , and Willingham, M.
Acfa351, 205-21 3. Keen, J. , Maxfield, F. , Hardegree, M. , and Habig, W. H. (1982). Proc. Acad. Sci. 79,2912-2916. 28 FREDERICK R. MAXFIELD Levitzki, A. (1985). In “Endocytosis” (I. Pastan and M. C. Willingham. ), pp. 45-68. Plenum, New York. Maxfield, F. R. (1985). In “Endocytosis” (I. Pastan and M. C. ), pp. 235 -257. Plenum, New York. Maxfield, F. , and Willingham, M. C. (1978). Cell 14,805-810. Maxfield, F. , Willingham, M. , Haigler, H. , and Pastan, I. (1981a). Biochemistry 20,5353-5358.