By Ludwig Eichinger
Dictyostelium discoideum Protocols provides a entire choice of state of the art molecular organic protocols for learning D. discoideum, with emphasis on its application within the examine of primary mobile tactics together with sign transduction, chemotaxis, phone motility, cytokinesis, phagocytosis, and facets of improvement reminiscent of telephone sorting, trend formation, and phone sort differentiation. Written by way of specialists within the box, this crucial consultant offers quick access to present equipment and strategies for the learn of D. discoideum. For the newcomer to the sphere, it bargains an entire and inclusive advent to this crucial version organism. The e-book is split into 4 major components, which come with an creation to the organism that offers necessary neighborhood assets and genome-wide methods; simple tools and to be had molecular genetic thoughts for research of the organism; imaging and localization equipment; and a dialogue of D. discoideum, with emphasis on its particular benefits as a version system.
This in-depth consultant offers an entire advent to and thorough guide for the research of D. discoideum, together with a dialogue of the consequences of the lately accomplished Dictyostelium genome undertaking in strengthening the location of D. discoideum as a version organism for learning primary cellphone strategies and elements of improvement. Researchers in any respect degrees of expertise will enjoy the displays all through of the most up-tp-date, worthy, and cutting edge recommendations for the examine of D. discoideum from major Dictyostelium scientists.
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Extra info for Dictyostelium discoideum Protocols
Example text
Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells. com Methods for Ultrastructural Studies of Dictyostelium discoideum. Part IV Dictyostelium as Model Organism. Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P 3 In Vivo and In Vitro. Assaying Chemotaxis of Dictyostelium Cells. Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light Scattering. Quantitative and Microscopic Methods for Studying the Endocytic Pathway.
Regulator of G protein signaling [RGS] proteins), cell cycle control, and domains involved in signaling. It also appears that glycogen storage and utilization arose (or was retained) as a metabolic strategy soon after the plant/animal divergence, because glycogen synthetase appears in this evolutionary interval. Also particularly striking are the cases in which otherwise ubiquitous Pfam domains appear to be completely absent in one group or another. For instance, Dictyostelium appears to have lost the genes that encode collagen domains and basic helix-loop-helix (bHLH) transcription factors.
Coverage is estimated to include at least 99% of the protein coding information. As one measure of the completeness of the genome sequence, 966 of 967 previously well characterized Dictyostelium genes were identified in the assembled sequence. The lengthy and challenging task of sequencing the genome has been worth the effort because it can now be used to characterize the structure of the chromosomes and the genetic information they carry. org (4). 2. The Chromosomes The nuclear genome of Dictyostelium consists of six chromosomes totaling 34 Mb and approx 100 copies of a linear 88-kb palindrome that carries genes for the ribosomal RNAs and no other functional genes (1,5).