By M. A. Hayat
Considering that its advent in 1971, the improvement and alertness of colloidal gold as a marker in electron microscopy has been exceptional. This state-of-the artwork, multi-volume treatise offers researchers, technicians, academics, and scholars with the main entire insurance of the rules and technique of colloidal gold microscopy on hand today.
This common procedure is appropriate to such a lot microscopical platforms together with optical microscopy, scanning, transmission and excessive voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold permits low and high solution reports, enzyme and nucleic acid labeling, learn of dynamic mobile tactics, and virus detection.
Topics coated in quantity 1 include:
* ideas, equipment, and purposes of colloidal gold method in cytochemistry and immunochemistry
* tools for getting ready colloidal gold debris of alternative sizes
* Protein A-gold, protein G-gold, and lectin-gold techniques
* using resins and skinny cryosections
* a number of labeling
Read Online or Download Colloidal Gold. Principles, Methods, and Applications PDF
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Extra resources for Colloidal Gold. Principles, Methods, and Applications
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Sei. A. 79, 7474. Zikan, J. (1980). Interactions of pig Fab gamma fragments with protein A from Staphylococ cus aureus. Folia Microbiol. (Prague) 25, 246. Zsigmondy, R. (1901). Z. Anal. Chem. 40, 697. Zsigmondy, R. (1905). Zur Erkenntnisse der Kolloide. Fischer, Jena. , and T h i e s s e n , P. A . (1925). Das kolloidale gold. Akad. Verlagsges. Leipzig. 3 Role of Tissue Processing in Colloidal Gold Methods GEOFFREY R. NEWMAN and J A N Α. ΗΟΒΟΤ Electron Microscopy Unit University of Wales College of Medicine Heath Park, Cardiff, Wales INTRODUCTION LR WHITE AND LOWICRYL K4M LR WHITE OR LOWICRYL K4M?
Cytochem. 3 2 , 705. Horisberger, M . , and Vauthey, M. (1984). Labelling of colloidal gold with protein. A quanti tative study using beta-lactoglobulin. Histochemistry 8 0 , 13. 32 Alex D. Hyat Hyatt, A . D . , Eaton, Β. T . , and Lunt, R. (1987). The grid-cell-culture technique: The direc examination of virus-infected cells and progeny viruses. J. Microsc. (Oxford) 145, 97 Hyatt, A . D . , M c P h e e , D . Α . , and White, J. R. (1988). Antibody competition studies witl gold-labelling immunoelectron microscopy.