By Theodore Kuwana
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Extra resources for Physical Methods in Modern Chemical Analysis. Volume 2
Sample text
High Performance Liquid Chromatography 39 Although inexpensive, this latter type of system does not generally offer the reproducibility provided by the commercial instrumentation. A recent useful design employs a single high pressure p u m p which switches from reservoir to reservoir by a switching valve according to a predetermined p r o g r a m selected by the operator. The solvents are mixed on the low pressure side in a mixing chamber prior to passage through the p u m p . This system can also be designed to produce ternary gradients.
If the sample enters the packed bed at the center of its cross section, lateral dispersion is given by W 2 = 2Ad z + (32DJU)Z p + W (51) 2 where W is the radial peak width, W the initial band dispersion, d the particle size, Ζ the distance traveled in the column at velocity U, and D the diffusion coefficient of the solute in the mobile phase. In Eq. (51) the first term is due to stream splitting and the second to molecular diffusion. A typical peak spread illustrating an unfavorable and favorable condition is shown in Fig.
If a low conducting mobile phase is used, such as pure solvent or mixed solvent in the absence of electrolytes, a greater sensitivity is experienced and often ap proaches that of the solute property detector (approximately 10 " g/ml). 8 6. Solute Property Detectors A solute property detector responds to a specific property of a solute in the mobile phase that the mobile phase does not possess or has to a significantly lesser extent. These types of detectors are the most used in H P L C at present.