By Geza Hrazdina (auth.), Helen A. Stafford, Ragai K. Ibrahim (eds.)
This quantity comprises studies provided on the 31 st annual assembly of the Phytochemical Society of North the US, held at Colorado nation collage in fortress Collins, Colorado on June 22-26, 1991. This symposium, entitled Phenolic Metabolism in crops, celebrated the foundation of this society because the Plant Phenolics workforce of North the USA; the 1st symposium, entitled Biochemistry of Plant Phenolic ingredients, used to be additionally held at fortress Collins from August 31 to September 1, 1961. a quick background of the Society is gifted in bankruptcy 12 via Stewart Brown, one of many unique founders of the Society. We devote this quantity to Hans Grisebach, 1926-1990, Professor of Biochemistry on the Biologisches Institut II, Freiburg, Germany, the place he headed for a few years a laboratory answerable for significant advances within the quarter of phenolic metabolism; it will be self glaring from the various bibliographical references pointed out within the literature for papers by means of his Freiburg workforce from approximately 1958 formerly, and therefore by means of former scholars and colla borators. His influence at the facts reviewed during this quantity will testify to this.
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USA 84:8966-8970. , WIERMANN, R. 1988. ). Planta 173:532-543. , HRAZDINA, G. 1991. Stereoisomerism in plant disease resistance: Induction and isolation of the 7,2'-dihydroxy-4' ,5'-methylenedioxyisoflavone oxidoreductase, an enzyme introducing chirality during synthesis of isoflavonoid phytoalexins in pea (Pisum salivum L). Arch. Biochem. Biophys. 284:167-173. LIPMAN, T. 1926. Die Anthocyanophore der Erythrea-Arten. Beitr. Bot. Zentralbl. Abt. 143:127-132. MOLLISCH, H. 1923. Mikruchemie der Pflanzen.
69 ,73 Immunofluorescence The indirect immunofluorescence technique is commonly used after the tissue is cryoprotected, embedded in glycol methacrylate (-30°C) and sectioned. The tissue is first incubated with the primary antibody. then washed to remove unbound antibodies. 6 In this technique, fixed tissues are embedded in plastic resin and ultrathin sections are first incubated with the unlabeled primary antibody, followed by incubation with either a second antibody or protein A, both of which are conjugated with colloidal gold particles.
Introduction.. ........... ........... Preparation of antigen.................................................................. Preparation of hapten-protein conjugates .......................................... Production of antisera... .............. ........... Immunocytochemistry. ........... ......... .................... Tissue fixation and embedding....................................................... Immunofluorescence....................................................................