By Jorge F. S. Ferreira, Jules Janick (auth.), Professor Dr. Toshiyuki Nagata, Professor Dr. Yutaka Ebizuka (eds.)
Medicinal and fragrant vegetation XII comprises 18 chapters. It offers with the distribution, value, traditional propagation, micropropagation, tissue tradition experiences, and the in vitro construction of significant medicinal and pharmaceutical compounds within the following vegetation: Artemisiaannua, Coriandrum sativum, Crataegus, Dionaea muscipula,Hyoscyamus reticulatus, Hypericum canariense, Leguminosae, Malva,Ocimum, Pergulariatomentosa, Phellodendron amurense,Sempervivum, Solanumaculeatissimum, S. chrysotrichum, S. kasianum, Stephania,Trigonella, and Vaccinium. it truly is adapted to the wishes of complex scholars, academics, and learn scientists within the fields of pharmacy, plant tissue tradition, phytochemistry, biomedical engineering, and plant biotechnology regularly.
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1979), or ethyl acetate/formic acid/water (8/1/1, v/v/v) for ftavonoids (Lamaison and Carnat 1991). Proanthocyanidins/catechins are revealed (orange to red, visible) with anisaldehyde-sulfuric reagent at 105°C, and ftavonoids at 365nm (yellow, orange, green) by 2-aminoethyldiphenylborate (1 %, methanolic) reagent followed by 5% methanolic polyethyleneglycol 4000. 1 Total Phenols Total phenols are measured in extract I according to Singleton et al. 5 ml Folin-Ciocalteu's reagent (Merck), then after 3 min mixed with 2 m120% Na2C03 After 1 min in a boiling water bath, absorption of the cooled solution is measured at 680 nm and compared to a gallic acid standard.
2 Phenols in Suspension Cultures Cell suspension cultures established after 2-year callus culture showed a much higher biomass production between days 24 and 32 with a 12-fold increase. The phenolic profile was similar to that in calluses except for flavonoid derivatives which were not detected (Table 2). 26gflOOg DW at day 32; Fig. 5). 23 g/IOO g) were produced in cell suspension cultures (Fig. 5). 687 gflOO g DW) was observed. 64gIl00g DW at day 32; Fig. 6). 77 gflOO g DW) than in calluses (Fig.
Our aim was to establish a long-term, high phenolic-producing tissue culture from hawthorn. The floral bud produced calluses which, during the first 63 weeks of their establishment, showed the formation of reddish areas. Due to prior experience in buckwheat tissue cultures, the presence of anthocyanins led us to expose the cultures to continuous light which induced higher phenolic synthesis. Calluses and suspension cultures showed similar phenolic profiles, especially for proanthocyanidins and catechins, except that cell suspensions produced very few anthocyanins and practically no flavonoids.