By Rodolfo Paoletti; David Kritchevsky
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Additional resources for Advances in lipid research. Vol. 18, 1981
We also used a similar technique for gross measurement of myocardial infarction after identifying the acute lesions by cardiac perfusion and stain ing with Nitroblue tetrazolium. All in all, point counting techniques are very useful, although slightly Pathology in Atherosclerosis Research 39 tedious, and without d o u b t they have their place in research concerned with atherosclerotic disease. , 1980). 5. Autoradiography Isotope-labeled substances are widely used in the study of atherogenesis, mainly t o study the degree of accumulation and incorporation of various substances suspected of playing a role in the disease process.
They have been used in an attempt to label endothelial cells, but they also react with platelets (Stemerman and Pitlick, 1974). Stemerman et al. (1976) used antibody t o tissue factor apoprotein a b sorbed with 7-globulin a n d coupled to horseradish peroxidase; this com plex was then applied t o various n o r m a l rabbit tissues and the distribution 36 DRAGOSLAVA VESSELINOVITCH AND KATTI F I S C H E R - D Z O G A of the peroxidase marker was observed by electron microscopy. They observed that only the endothelial cells that had been exposed t o the an tibody complex showed localization of the electron-dense reaction product.
Enzyme Histochemistry. Enzyme histochemical staining methods are 28 DRAGOSLAVA VESSELINOVITCH AND K A T T I F I S C H E R - D Z O G A also employed in the study of atherosclerosis and they are useful in studies concerned with lysosomal enzymes such as acid phosphatase, ^-glucuron idase, and acid lipase or cholesteryl esterase. Acid phosphatase is usually demonstrated with light microscopy by a modification of Lojda's method (Pearse, 1968) which is a diazo coupling method with naphthol-AS-BIphosphate as a substrate, and hexazonium pararosanilin as coupler.