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Enzymes in Lipid Modification by Uwe Theo Bornscheuer, DGF

25 February 2017 adminGeneral Reference

By Uwe Theo Bornscheuer, DGF

Enzymatic tools of lipid amendment, relatively of fat and oils, have built quickly because the Eighties. In parallel to the quick development in examine a variety of purposes have emerged, e.g. within the meals industry.The ebook is written by way of top specialists within the box and displays the state of the art of enzymatic lipid amendment. It presents the reader with guidance easy methods to pick out appropriate enzymes and the way to use them efficiently.Applications of lipases and phospholipases, lipoxygenases and P450-monooxygenases and using whole-cell structures in lipid amendment are defined. Cloning, expression and mutagenesis in addition to makes an attempt to appreciate the molecular foundation of specificity and stereoselectivity are defined. furthermore engineering points and the alternative of solvent structures are addressed.

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Extra info for Enzymes in Lipid Modification

Sample text

Mattiasson, B. (1994), Lipase-catalyzed alcoholysis of triglycerides for the preparation of 2-monoglycerides, Enzyme Microb. Technol. 16, 1042 – 1047. , Mattiasson, B. (1996), Glyceride synthesis in a solvent free system, J. Am. Oil Chem. Soc. 73, 11, 1489 – 1495. , Mattiasson, B. (1997), Preparation of diglycerides by lipase-catalyzed alcoholysis of triglycerides, Enzyme Microb. Technol. 20, 198 – 206. P. , J. Am. Oil Chem. Soc. 73, 1409 – 1414. J. B. (Eds), Lipases; their Structure, Biochemistry and Application, Cambridge University Press, pp.

However, with R. miehei lipase as biocatalyst selective interesterification of tuna oil triacylglycerols with ethanol yields an acylglycerol fraction containing 49 % n-3 22 : 6, whereas selective esterification of tuna oil fatty acids with ethanol yields an unesterified fatty acid fraction containing 74 % n-3 22 : 6 (Haraldsson and Kristinsson, 1998). , 1981), nervonic (cis-15-tetracosenoic, 24 : 1) acid (Mukherjee and Kiewitt, 1986) and cis-5-eicosenoic acid (Joliff, 1981) are abundant constituents of seed oils, especially from Cruciferae.

Continued Lipase from Reaction Specificity Reference R. , 1995 C. rugosa Hydrolysis of borage oil Discrimination against n-6 18 : 3 Ergan, 1996 R. , 1997 R. , 1997b C. rugosa, Pseudomonas cepacia, porcine pancreas Esterification of acetylenic fatty acids with n-butanol Preference for 10-undecynoic acid and strong discrimination against 6-octadecynoic acid Lie Ken Jie and Xun, 1998a C. rugosa, R. miehei, porcine pancreas Esterification of medium- and longchain olefinic and acetylenic alcohols with pentanoic acid and stearic acid Discrimination against Lie Ken Jie and Xun, medium- and long-chain 1998b olefinic alcohols in the esterification with pentanoic acid; olefinic and acetylenic alcohols well accepted in the esterification with stearic acid catalyzed by lipase from porcine pancreas Burkholderia sp.

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